| RIPK5

Dual serine/threonine and tyrosine protein kinase is an enzyme that is encoded by the DSTYK gene.This protein is also known as the Dusty protein kinase and the Receptor interacting protein 5 (RIP5).This gene encodes a dual serine/threonine and tyrosine protein kinase which is expressed in multiple tissues. Multiple alternatively spliced transcript variants have been found, but the biological validity of some variants has not been determined.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.37	1.58	1.01	0.65	0.43	0.31	0.22	0.09
Corrected OD	2.28	1.49	0.92	0.56	0.34	0.22	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat RIPK5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	91-105	102

 

| Linearity

Samples were spiked with high concentrations of Rat RIPK5 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	87-96
	Average (%)	93
1 : 4	Range (%)	87-108
	Average (%)	98